Vivekananda Institute of Biotechnology

Science in the Service of Mankind

Botanical Pesticide

 zNeem tree can control more than 200 insect species and some nematodes, fungi, bacteria and iruses. Neem acts differently on pest.It does not kill pest, it contains several active chemicals (Azadirachtine, Salannin, Gedunin etc) which change the insect’s food habits or life cycle. So it is safe for the man, animal and environment.

Neem based pesticides are suitable for developing countries because the useful chemical can be extracted easily without any expensive equipment. The left over can improve soil and can be added as plant food. VIB promots use extract from kernel, oil and cake.

It has been estimated that 20 to 30kg of neem seed (an average yield from 2 trees), prepared as kernel extract can treat one hectare of crop. This is very effective against cutworms affected plants like many vegetables, maize, tobacco and coffee.

Production of neem seed kernel extract and its application were standardized for different crops of this region. The methodology involves collection ripe neem fruits, de-pulping of the seeds and drying in shade, decortications of dried seeds, grinding and sieving of kernel, preparation of aquatic extract, and spraying on the cop plants. Field demonstration with NSKE is going on at different regions of Sunderbans under a project funded by Ministry of Fertilizers and Chemicals, Govt. of India.

Preparation of Neem Seed Kernal Aqueous Extract  

 Step –I : Collection of yellow ripe fruits



Step –II : De-pulping and washing of ripe fruits

Step –III : Shade drying of De-pulped seeds 

Step –IV : Grinding of seeds by Mortar and Pestle

 Step –V : Sieving of powder seeds by 18 mesh sieve

Step –VI : Soaking of powdered seed in water for six hours

Step –VII : Straining of soaked seed through muslin cloths

Step –VIII : Filling up sprayer with Neem Seed Kernel Extract

Step –IX : Spraying Neem Seed Kernel Extract on field crop by sprayer








Biological control of pest and disease is an important component of modern day agricultute and to achieve this, a laboratory set up is developed for the maintenance of germplasms of different biological control agents, development of rurally adaptable, scientific and low cost production method.

Germplasm: Trichoderma, Bacillus thuringensis, Metarrhizium, Beauveria, Paecilomyces and Trichogama